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Rational elicitation of cold-sensitive phenotypes

Baliga, Chetana and Majhi, Sandipan and Mondal, Kajari and Bhattacharjee, Antara and VijayRaghavan, K and Varadarajan, Raghavan (2016) Rational elicitation of cold-sensitive phenotypes. In: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 113 (18). E2506-E2515.

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Official URL: http://dx.doi.org/10.1073/pnas.1604190113

Abstract

Cold-sensitive phenotypes have helped us understand macromolecular assembly and biological phenomena, yet few attempts have been made to understand the basis of cold sensitivity or to elicit it by design. We report a method for rational design of cold-sensitive phenotypes. The method involves generation of partial loss-of-function mutants, at either buried or functional sites, coupled with selective overexpression strategies. The only essential input is amino acid sequence, although available structural information can be used as well. The method has been used to elicit cold-sensitive mutants of a variety of proteins, both monomeric and dimeric, and in multiple organisms, namely Escherichia coli, Saccharomyces cerevisiae, and Drosophila melanogaster. This simple, yet effective technique of inducing cold sensitivity eliminates the need for complex mutations and provides a plausible molecular mechanism for eliciting cold-sensitive phenotypes.

Item Type: Journal Article
Additional Information: Copy right for this article belongs to the NATL ACAD SCIENCES, 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA
Keywords: conditional mutants; rational design; cold sensitivity; heat-induced expression; transfer between organisms
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Depositing User: Id for Latest eprints
Date Deposited: 17 Jun 2016 05:16
Last Modified: 17 Jun 2016 05:16
URI: http://eprints.iisc.ac.in/id/eprint/53959

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