Ganguly, Abira and Bhattacharjee, Chumki and Bhave, Madhura and Kailaje, Vaishali and Jain, Bhawik K and Sengupta, Isha and Rangarajan, Annapoorni and Bhattacharyya, Dibyendu (2016) Perturbation of nucleo-cytoplasmic transport affects size of nucleus and nucleolus in human cells. In: FEBS LETTERS, 590 (5). pp. 631-643.
Full text not available from this repository. (Request a copy)Abstract
Size regulation of human cell nucleus and nucleolus are poorly understood subjects. 3D reconstruction of live image shows that the karyoplasmic ratio (KR) increases by 30-80% in transformed cell lines compared to their immortalized counterpart. The attenuation of nucleo-cytoplasmic transport causes the KR value to increase by 30-50% in immortalized cell lines. Nucleolus volumes are significantly increased in transformed cell lines and the attenuation of nucleo-cytoplasmic transport causes a significant increase in the nucleolus volume of immortalized cell lines. A cytosol and nuclear fraction swapping experiment emphasizes the potential role of unknown cytosolic factors in nuclear and nucleolar size regulation.
| Item Type: | Journal Article |
|---|---|
| Publication: | FEBS LETTERS |
| Publisher: | WILEY-BLACKWELL |
| Additional Information: | Copy right for this article belongs to the WILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA |
| Keywords: | cancer; importin beta; karyoplasmic ratio; nuclear transport; nucleolus size; nucleus size; organelle size; Ran |
| Department/Centre: | Division of Biological Sciences > Molecular Reproduction, Development & Genetics |
| Date Deposited: | 02 Apr 2016 05:31 |
| Last Modified: | 02 Apr 2016 05:31 |
| URI: | http://eprints.iisc.ac.in/id/eprint/53585 |
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