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Perturbation of nucleo-cytoplasmic transport affects size of nucleus and nucleolus in human cells

Ganguly, Abira and Bhattacharjee, Chumki and Bhave, Madhura and Kailaje, Vaishali and Jain, Bhawik K and Sengupta, Isha and Rangarajan, Annapoorni and Bhattacharyya, Dibyendu (2016) Perturbation of nucleo-cytoplasmic transport affects size of nucleus and nucleolus in human cells. In: FEBS LETTERS, 590 (5). pp. 631-643.

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Official URL: http://dx.doi.org/10.1002/1873-3468.12077

Abstract

Size regulation of human cell nucleus and nucleolus are poorly understood subjects. 3D reconstruction of live image shows that the karyoplasmic ratio (KR) increases by 30-80% in transformed cell lines compared to their immortalized counterpart. The attenuation of nucleo-cytoplasmic transport causes the KR value to increase by 30-50% in immortalized cell lines. Nucleolus volumes are significantly increased in transformed cell lines and the attenuation of nucleo-cytoplasmic transport causes a significant increase in the nucleolus volume of immortalized cell lines. A cytosol and nuclear fraction swapping experiment emphasizes the potential role of unknown cytosolic factors in nuclear and nucleolar size regulation.

Item Type: Journal Article
Additional Information: Copy right for this article belongs to the WILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
Keywords: cancer; importin beta; karyoplasmic ratio; nuclear transport; nucleolus size; nucleus size; organelle size; Ran
Department/Centre: Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Depositing User: Id for Latest eprints
Date Deposited: 02 Apr 2016 05:31
Last Modified: 02 Apr 2016 05:31
URI: http://eprints.iisc.ac.in/id/eprint/53585

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