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DNA methylation analysis of phenotype specific stratified Indian population

Rotti, Harish and Mallya, Sandeep and Kabekkodu, Shama Prasada and Chakrabarty, Sanjiban and Bhale, Sameer and Bharadwaj, Ramachandra and Bhat, Balakrishna K and Dedge, Amrish P and Dhumal, Vikram Ram and Gangadharan, GG and Gopinath, Puthiya M and Govindaraj, Periyasamy and Joshi, Kalpana S and Kondaiah, Paturu and Nair, Sreekumaran and Nair, Venugopalan SN and Nayak, Jayakrishna and Prasanna, BV and Shintre, Pooja and Sule, Mayura and Thangaraj, Kumarasamy and Patwardhan, Bhushan and Valiathan, Marthanda Varma Sankaran and Satyamoorthy, Kapaettu (2015) DNA methylation analysis of phenotype specific stratified Indian population. In: JOURNAL OF TRANSLATIONAL MEDICINE, 13 .

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Official URL: http://dx.doi.org/10.1186/s12967-015-0506-0

Abstract

Background: DNA methylation and its perturbations are an established attribute to a wide spectrum of phenotypic variations and disease conditions. Indian traditional system practices personalized medicine through indigenous concept of distinctly descriptive physiological, psychological and anatomical features known as prakriti. Here we attempted to establish DNA methylation differences in these three prakriti phenotypes. Methods: Following structured and objective measurement of 3416 subjects, whole blood DNA of 147 healthy male individuals belonging to defined prakriti (Vata, Pitta and Kapha) between the age group of 20-30years were subjected to methylated DNA immunoprecipitation (MeDIP) and microarray analysis. After data analysis, prakriti specific signatures were validated through bisulfite DNA sequencing. Results: Differentially methylated regions in CpG islands and shores were significantly enriched in promoters/UTRs and gene body regions. Phenotypes characterized by higher metabolism (Pitta prakriti) in individuals showed distinct promoter (34) and gene body methylation (204), followed by Vata prakriti which correlates to motion showed DNA methylation in 52 promoters and 139 CpG islands and finally individuals with structural attributes (Kapha prakriti) with 23 and 19 promoters and CpG islands respectively. Bisulfite DNA sequencing of prakriti specific multiple CpG sites in promoters and 5'-UTR such as; LHX1 (Vata prakriti), SOX11 (Pitta prakriti) and CDH22 (Kapha prakriti) were validated. Kapha prakriti specific CDH22 5'-UTR CpG methylation was also found to be associated with higher body mass index (BMI). Conclusion: Differential DNA methylation signatures in three distinct prakriti phenotypes demonstrate the epigenetic basis of Indian traditional human classification which may have relevance to personalized medicine.

Item Type: Journal Article
Additional Information: Copy right for this article belongs to the BIOMED CENTRAL LTD, 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND
Keywords: DNA methylation; MeDIP; CpG Island; Prakriti; Ayurveda
Department/Centre: Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Depositing User: Id for Latest eprints
Date Deposited: 03 Mar 2016 05:40
Last Modified: 03 Mar 2016 05:40
URI: http://eprints.iisc.ac.in/id/eprint/53378

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