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Identification of microRNAs and their targets in Finger millet by high throughput sequencing

Usha, S and Jyothi, MN and Sharadamma, N and Dixit, Rekha and Devaraj, VR and Babu, Nagesh R (2015) Identification of microRNAs and their targets in Finger millet by high throughput sequencing. In: GENE, 574 (2). pp. 210-216.

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Official URL: http://dx.doi.org/10.1016/j.gene.2015.08.007

Abstract

MicroRNAs are short non-coding RNAs which play an important role in regulating gene expression by mRNA cleavage or by translational repression. The majority of identified miRNAs were evolutionarily conserved; however, others expressed in a species-specific manner. Finger millet is an important cereal crop; nonetheless, no practical information is available on microRNAs to date. In this study, we have identified 95 conserved microRNAs belonging to 39 families and 3 novel microRNAs by high throughput sequencing. For the identified conserved and novel miRNAs a total of 507 targets were predicted. 11 miRNAs were validated and tissue specificity was determined by stem loop RT-qPCR, Northern blot. GO analyses revealed targets of miRNA were involved in wide range of regulatory functions. This study implies large number of known and novel miRNAs found in Finger millet which may play important role in growth and development. (C) 2015 Elsevier B.V. All rights reserved.

Item Type: Journal Article
Additional Information: Copy right for this article belongs to the ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
Keywords: cis-regulatory motifs; GO analysis; RT-qPCR; RISC; TFBs; TSS
Department/Centre: Division of Biological Sciences > Biochemistry
Depositing User: Id for Latest eprints
Date Deposited: 15 Dec 2015 07:44
Last Modified: 15 Dec 2015 07:44
URI: http://eprints.iisc.ac.in/id/eprint/52904

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