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Production and preliminary evaluation of Trypanosoma evansi HSP70 for antibody detection in Equids

Kumar, Jaideep and Chaudhury, Ashok and Bera, Bidhan C and Kumar, Ritesh and Kumar, Rajender and Tatu, Utpal and Yadav, Suresh Chandra (2015) Production and preliminary evaluation of Trypanosoma evansi HSP70 for antibody detection in Equids. In: ACTA PARASITOLOGICA, 60 (4). pp. 727-734.

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Official URL: http://dx.doi.org/10.1515/ap-2015-0104

Abstract

The present immuno-diagnostic method using soluble antigens from whole cell lysate antigen for trypanosomosis have certain inherent problems like lack of standardized and reproducible antigens, as well as ethical issues due to in vivo production, that could be alleviated by in vitro production. In the present study we have identified heat shock protein 70 (HSP70) from T. evansi proteome. The nucleotide sequence of T. evansi HSP70 was 2116 bp, which encodes 690 amino acid residues. The phylogenetic analysis of T. evansi HSP70 showed that T. evansi occurred within Trypanosoma clade and is most closely related to T. brucei brucei and T. brucei gambiense, whereas T. congolense HSP70 laid in separate clade. The two partial HSP70 sequences (HSP-1 from N-terminal region and HSP-2 from C-terminal region) were expressed and evaluated as diagnostic antigens using experimentally infected equine serum samples. Both recombinant proteins detected antibody in immunoblot using serum samples from experimental infected donkeys with T. evansi. Recombinant HSP-2 showed comparable antibody response to Whole cell lysate (WCL) antigen in immunoblot and ELISA. The initial results indicated that HSP70 has potential to detect the T. evansi infection and needs further validation on large set of equine serum samples.

Item Type: Journal Article
Additional Information: Copy right for this article belongs to the WALTER DE GRUYTER GMBH, GENTHINER STRASSE 13, D-10785 BERLIN, GERMANY
Keywords: Trypanosoma evansi; Sero-prevalence; Recombinant heat shock protein 70; Phylogenetic tree; Immunoblot; ELISA
Department/Centre: Division of Biological Sciences > Biochemistry
Depositing User: Id for Latest eprints
Date Deposited: 31 Oct 2015 05:54
Last Modified: 31 Oct 2015 05:54
URI: http://eprints.iisc.ac.in/id/eprint/52627

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