Field-Portable Microfluidics-Based Imaging Flow Cytometer

Jagannadh, Veerendra Kalyan and Murthy, Rashmi Sreeramachandra and Srinivasan, Rajesh and Gorthi, Sai Siva (2015) Field-Portable Microfluidics-Based Imaging Flow Cytometer. In: JOURNAL OF LIGHTWAVE TECHNOLOGY, 33 (16). pp. 3469-3474.

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Abstract

Clinical microscopy is a versatile and robust tool used for the diagnosis of a plethora of diseases. However, due to various reasons, it remains inaccessible in resource limited settings. In this paper, we present an automated and cost-effective alternative to microscopy for use in clinical diagnostics. With the use of custom optics and microfluidics, we demonstrate a field-portable imaging flow cytometry system. Using the presented system, we have been able to image 586 cells per second. We demonstrate the clinical relevance of the proposed system by differentiating between suspensions of healthy and sphered RBCs based on high-throughput morphometric analysis. The instrument presented here is a major advancement in the domain of field portable diagnostics as it enables fast and robust quantitative diagnostic testing at the point-of-care.

Item Type:	Journal Article
Publication:	JOURNAL OF LIGHTWAVE TECHNOLOGY
Publisher:	IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
Additional Information:	IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC, 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA
Keywords:	Cytometry; high-throughput imaging; microfluidics; morphological analysis; point-of-care diagnostics; portable microscopy
Department/Centre:	Division of Physical & Mathematical Sciences > Instrumentation Appiled Physics
Date Deposited:	11 Sep 2015 04:38
Last Modified:	11 Sep 2015 04:38
URI:	http://eprints.iisc.ac.in/id/eprint/52319

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