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Automated cell viability assessment using a microfluidics based portable imaging flow analyzer

Jagannadh, Veerendra Kalyan and Adhikari, Jayesh Vasudeva and Gorthi, Sai Siva (2015) Automated cell viability assessment using a microfluidics based portable imaging flow analyzer. In: BIOMICROFLUIDICS, 9 (2).

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Official URL: http://dx.doi.org/10.1063/1.4919402

Abstract

In this work, we report a system-level integration of portable microscopy and microfluidics for the realization of optofluidic imaging flow analyzer with a throughput of 450 cells/s. With the use of a cellphone augmented with off-the-shelf optical components and custom designed microfluidics, we demonstrate a portable optofluidic imaging flow analyzer. A multiple microfluidic channel geometry was employed to demonstrate the enhancement of throughput in the context of low frame-rate imaging systems. Using the cell-phone based digital imaging flow analyzer, we have imaged yeast cells present in a suspension. By digitally processing the recorded videos of the flow stream on the cellphone, we demonstrated an automated cell viability assessment of the yeast cell population. In addition, we also demonstrate the suitability of the system for blood cell counting. (C) 2015 AIP Publishing LLC.

Item Type: Journal Article
Additional Information: Copy right for this article belongs to the AMER INST PHYSICS, 1305 WALT WHITMAN RD, STE 300, MELVILLE, NY 11747-4501 USA
Keywords: PHYTOPLANKTON ANALYSIS; MICROSCOPY; CYTOMETRY; FIELD; MICROORGANISMS; PHONE
Department/Centre: Division of Physical & Mathematical Sciences > Instrumentation Appiled Physics
Depositing User: Id for Latest eprints
Date Deposited: 29 May 2015 06:36
Last Modified: 29 May 2015 06:36
URI: http://eprints.iisc.ac.in/id/eprint/51615

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