ePrints@IIScePrints@IISc Home | About | Browse | Latest Additions | Advanced Search | Contact | Help

MRT Letter: A Unified Accelerated Maximum Likelihood Technique for Widefield, Confocal, and Super-Resolution 4Pi Microscopy

Chelur, Rasmi K and Kanhirodan, Rajan and Mondal, Partha Pratim (2015) MRT Letter: A Unified Accelerated Maximum Likelihood Technique for Widefield, Confocal, and Super-Resolution 4Pi Microscopy. In: MICROSCOPY RESEARCH AND TECHNIQUE, 78 (5). pp. 331-335.

[img] PDF
mic_res_tec-78_5_331_2015.pdf - Published Version
Restricted to Registered users only

Download (462kB) | Request a copy
Official URL: http://dx.doi.org/10.1002/jemt.22479

Abstract

We propose an algorithmic technique for accelerating maximum likelihood (ML) algorithm for image reconstruction in fluorescence microscopy. This is made possible by integrating Biggs-Andrews (BA) method with ML approach. The results on widefield, confocal, and super-resolution 4Pi microscopy reveal substantial improvement in the speed of 3D image reconstruction (the number of iterations has reduced by approximately one-half). Moreover, the quality of reconstruction obtained using accelerated ML closely resembles with nonaccelerated ML method. The proposed technique is a step closer to realize real-time reconstruction in 3D fluorescence microscopy. Microsc. Res. Tech. 78:331-335, 2015. (c) 2015 Wiley Periodicals, Inc.

Item Type: Journal Article
Additional Information: Copy right for this article belongs to the WILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
Keywords: imaging; image reconstruction; fluorescence microscopy
Department/Centre: Division of Physical & Mathematical Sciences > Instrumentation Appiled Physics
Division of Physical & Mathematical Sciences > Physics
Depositing User: Id for Latest eprints
Date Deposited: 22 May 2015 07:20
Last Modified: 22 May 2015 07:20
URI: http://eprints.iisc.ac.in/id/eprint/51557

Actions (login required)

View Item View Item