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Efficient generation of diffraction-limited multi-sheet pattern for biological imaging

Mondal, Partha Pratim and Dilipkumar, Shilpa and Mohan, Kavya (2015) Efficient generation of diffraction-limited multi-sheet pattern for biological imaging. In: OPTICS LETTERS, 40 (4). pp. 609-612.

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Official URL: http://dx.doi.org/ 10.1364/OL.40.000609

Abstract

We demonstrate a new technique to generate multiple light-sheets for fluorescence microscopy. This is possible by illuminating the cylindrical lens using multiple copies of Gaussian beams. A diffraction grating placed just before the cylindrical lens splits the incident Gaussian beam into multiple beams traveling at different angles. Subsequently, this gives rise to diffraction-limited light-sheets after the Gaussian beams pass through the combined cylindrical lens-objective sub-system. Direct measurement of field at and around the focus of objective lens shows multi-sheet pattern with an average thickness of 7.5 mu m and inter-sheet separation of 380 mu m. Employing an independent orthogonal detection sub-system, we successfully imaged fluorescently-coated yeast cells (approximate to 4 mu m) encaged in agarose gel-matrix. Such a diffraction-limited sheet-pattern equipped with dedicated detection system may find immediate applications in the field of optical microscopy and fluorescence imaging. (C) 2015 Optical Society of America

Item Type: Journal Article
Additional Information: Copy right for this article belongs to the OPTICAL SOC AMER, 2010 MASSACHUSETTS AVE NW, WASHINGTON, DC 20036 USA
Keywords: LIGHT SHEET MICROSCOPY; PLANE ILLUMINATION MICROSCOPY; FLUORESCENCE MICROSCOPY; SCANNING-MICROSCOPE; OPTICAL NANOSCOPY; HIGH-RESOLUTION; MOUSE-BRAIN; RECONSTRUCTION; FIELD; BEAM
Department/Centre: Division of Physical & Mathematical Sciences > Instrumentation Appiled Physics
Depositing User: Id for Latest eprints
Date Deposited: 20 Apr 2015 07:18
Last Modified: 20 Apr 2015 07:18
URI: http://eprints.iisc.ac.in/id/eprint/51244

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