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Serum proteomics of hepatitis C virus infection reveals retinol-binding protein 4 as a novel regulator

Gouthamchandra, K and Kumar, Anuj and Shwetha, Shivaprasad and Mukherjee, Anirban and Chandra, Madhavi and Ravishankar, B and Khaja, MN and Sadhukhan, Provash Chandra and Das, Saumitra (2014) Serum proteomics of hepatitis C virus infection reveals retinol-binding protein 4 as a novel regulator. In: JOURNAL OF GENERAL VIROLOGY, 95 (8). pp. 1654-1667.

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Official URL: http://dx.doi.org/ 10.1099/vir.0.062430-0

Abstract

Persistent infection of hepatitis C virus (HCV) can lead to liver cirrhosis and hepatocellular carcinoma, which are currently diagnosed by invasive liver biopsy. Approximately 15-20% of cases of chronic liver diseases in India are caused by HCV infection. In North India, genotype 3 is predominant, whereas genotype 1 is predominant in southern parts of India. The aim of this study was to identify differentially regulated serum proteins in HCV-infected Indian patients (genotypes 1 and 3) using a two-dimensional electrophoresis approach. We identified eight differentially expressed proteins by MS. Expression levels of one of the highly upregulated proteins, retinol-binding protein 4 (RBP4), was validated by ELISA and Western blotting in two independent cohorts. We also confirmed our observation in the JFH1 infectious cell culture system. Interestingly, the HCV core protein enhanced RBP4 levels and partial knockdown of RBP4 had a positive impact on HCV replication, suggesting a possible role for this cellular protein in regulating HCV infection. Analysis of RBP4-interacting partners using a bioinformatic approach revealed novel insights into the possible involvement of RBP4 in HCV-induced pathogenesis. Taken together, this study provided information on the proteome profile of the HCV-infected Indian population, and revealed a link between HCV infection, RBP4 and insulin resistance.

Item Type: Journal Article
Additional Information: Copy right for this article belongs to the
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Depositing User: Id for Latest eprints
Date Deposited: 10 Oct 2014 09:13
Last Modified: 10 Oct 2014 09:13
URI: http://eprints.iisc.ac.in/id/eprint/49983

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