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Biomediated separation of kaolinite and hematite using Bacillus subtilis

Poorni, S and Natarajan, KA (2013) Biomediated separation of kaolinite and hematite using Bacillus subtilis. In: 20th International Biohydrometallurgy Symposium (IBS 2013), OCT 08-11, 2013, Antofagasta, CHILE, pp. 223-226.

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Official URL: http://dx.doi.org/10.4028/www.scientific.net/AMR.8...

Abstract

Bacillus subtilis was used to demonstrate microbially induced selective flocculation to separate kaolinite and hematite. In neutral pH range of 7 - 8, 90 - 95% of hematite was selectively flocculated whereas 80 - 85% of kaolinite was dispersed using hematite - grown cells. Hematite-grown cells exhibited significant adsorption onto hematite than onto kaolinite, compared to unadapted cells. Kaolinite grown Bacillus subtilis secreted significant amounts of mineral specific proteins which conferred surface hydrophobicity whereas hematite-grown cells secreted more polysaccharides rendering hematite hydrophilic. Bacterial extracellular protein (EP) was isolated and the protein profiles of bacteria grown in the absence and presence of minerals were established.

Item Type: Conference Proceedings
Additional Information: copyright for this article belongs to TRANS TECH PUBLICATIONS LTD, LAUBLSRUTISTR 24, CH-8717 STAFA-ZURICH, SWITZERLAND
Keywords: Biobeneficiation; Bacillus subtilis; flocculation; hematite; kaolinite
Department/Centre: Division of Mechanical Sciences > Materials Engineering (formerly Metallurgy)
Depositing User: Id for Latest eprints
Date Deposited: 24 Jun 2014 10:33
Last Modified: 24 Jun 2014 10:33
URI: http://eprints.iisc.ac.in/id/eprint/49341

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