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Sequestration of the abrin A chain to the nucleus by BASP1 increases the resistance of cells to abrin toxicity

Gadadhar, Sudarshan and Bora, Namrata and Tiwari, Vinita and Karande, Anjali A (2014) Sequestration of the abrin A chain to the nucleus by BASP1 increases the resistance of cells to abrin toxicity. In: BIOCHEMICAL JOURNAL, 458 (2). pp. 375-385.

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Official URL: http://dx.doi.org/10.1042/BJ20131110

Abstract

Abrin, a type II ribosome-inactivating protein, comprises A and B subunits wherein the A subunit harbours toxin activity and the B subunit has a galactose-specific lectin activity. The entry of the protein inside the cell is through the binding of the B chain to cell surface glycoproteins followed by receptor-mediated endocytosis and retrograde transport. A previous study from our laboratory showed that different cell lines exhibited differences of as great as similar to 200-fold in abrin toxicity, prompting the present study to compare the trafficking of the toxin within cells. Observations made in this regard revealed that the abrin A chain, after being released into the cytosol, is sequestered into the nucleus through interaction with a cellular protein of similar to 25 kDa, BASP1 (brain acid-soluble protein 1). The nuclear localization of the A chain is seen predominantly in cells that are less sensitive to abrin toxicity and dependent on the levels of BASP1 in cells. The sequestration by BASP1 renders cells increasingly resistant to the inhibition of protein synthesis by abrin and the nucleus act as a sink to overcome cellular stress induced

Item Type: Journal Article
Additional Information: Copyright for this article belongs to the PORTLAND PRESS LTD, ENGLAND
Keywords: abrin; brain acid-soluble protein (BASP); intracellular trafficking; nuclear localization; ribosome-inactivating proteins
Department/Centre: Division of Biological Sciences > Biochemistry
Depositing User: Id for Latest eprints
Date Deposited: 16 May 2014 12:12
Last Modified: 16 May 2014 12:12
URI: http://eprints.iisc.ac.in/id/eprint/49045

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