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Basic studies on the role of components of Bacillus megaterium as flotation biocollectors in sulphide mineral separation

Vasanthakumar, Balasubramanian and Ravishankar, Honnavar and Subramanian, Sankaran (2014) Basic studies on the role of components of Bacillus megaterium as flotation biocollectors in sulphide mineral separation. In: APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 98 (6). pp. 2719-2728.

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Official URL: http://dx.doi.org/10.1007/s00253-013-5251-9

Abstract

Studies were carried out to assess the utility of the cellular and extracellular constituents of Bacillus megaterium for the flotation of sphalerite and galena minerals. Based on the flotation results on the individual minerals, it was observed that sphalerite was preferentially floated compared to galena. A maximum selectivity index (SI) value of 11.7 was achieved in the presence of the soluble fraction of the thermolysed cells, which was higher than that obtained with the intact cells (SI of 6.5) and the insoluble fraction of the thermolysed cells (SI of 9.6). The results of the various enzymatic treatment tests revealed that extracellular DNA played a vital role in the selective flotation of sphalerite. A noteworthy finding was that the single-stranded DNA (ssDNA) had a higher biocollector capacity vis-A -vis the double-stranded DNA (dsDNA), leading to better flotation efficiency. About 95 % recovery of sphalerite could be achieved from the mineral mixture by the combined addition of the ssDNA with the non-DNA components of the bacterial cells, resulting in a maximum SI of 19.1. Calcium and phosphate components of the nutrient media were found to be essential for better selectivity of separation of sphalerite. The mechanisms of microbe-mineral interaction are discussed.

Item Type: Journal Article
Additional Information: Copyright for this article belongs to the SPRINGER,USA
Keywords: Bacillus megaterium; DNA; Sphalerite; Galena; Bioflotation
Department/Centre: Division of Mechanical Sciences > Materials Engineering (formerly Metallurgy)
Depositing User: Id for Latest eprints
Date Deposited: 11 Apr 2014 09:50
Last Modified: 11 Apr 2014 09:50
URI: http://eprints.iisc.ac.in/id/eprint/48829

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