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In Vivo Genotoxicity Assessment of Titanium Dioxide Nanoparticles by Allium cepa Root Tip Assay at High Exposure Concentrations

Pakrashi, Sunandan and Jain, Nitin and Dalai, Swayamprava and Jayakumar, Jerobin and Chandrasekaran, Prathna Thanjavur and Raichur, Ashok M. and Chandrasekaran, Natarajan and Mukherjee, Amitava (2014) In Vivo Genotoxicity Assessment of Titanium Dioxide Nanoparticles by Allium cepa Root Tip Assay at High Exposure Concentrations. In: PLOS ONE, 9 (2).

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Official URL: http://dx.doi.org/10.1371/journal.pone.0087789

Abstract

The industrial production and commercial applications of titanium dioxide nanoparticles have increased considerably in recent times, which has increased the probability of environmental contamination with these agents and their adverse effects on living systems. This study was designed to assess the genotoxicity potential of TiO2 NPs at high exposure concentrations, its bio-uptake, and the oxidative stress it generated, a recognised cause of genotoxicity. Allium cepa root tips were treated with TiO2 NP dispersions at four different concentrations (12.5, 25, 50, 100 mu g/mL). A dose dependant decrease in the mitotic index (69 to 21) and an increase in the number of distinctive chromosomal aberrations were observed. Optical, fluorescence and confocal laser scanning microscopy revealed chromosomal aberrations, including chromosomal breaks and sticky, multipolar, and laggard chromosomes, and micronucleus formation. The chromosomal aberrations and DNA damage were also validated by the comet assay. The bio-uptake of TiO2 in particulate form was the key cause of reactive oxygen species generation, which in turn was probably the cause of the DNA aberrations and genotoxicity observed in this study.

Item Type: Journal Article
Additional Information: copyright for this article belongs to the authors.
Department/Centre: Division of Mechanical Sciences > Materials Engineering (formerly Metallurgy)
Depositing User: Id for Latest eprints
Date Deposited: 25 Mar 2014 09:34
Last Modified: 25 Mar 2014 09:35
URI: http://eprints.iisc.ac.in/id/eprint/48629

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