Kumar, Alok and Webster, Thomas J and Biswas, Krishanu and Basu, Bikramjit (2013) Flow cytometry analysis of human fetal osteoblast fate processes on spark plasma sintered hydroxyapatite-titanium biocomposites. In: Journal of Biomedical Materials Research Part A, 101 (10). pp. 2925-2938.
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Hydroxyapatite (HA)-based biocomposites have been widely investigated for a multitude of applications and these studies have been largely driven to improve mechanical properties (toughness and strength) without compromising cytocompatibility properties. Apart from routine cell viability/proliferation analysis, limited efforts have been made to quantify the fate processes (cell proliferation, cell cycle, and cell apoptosis) of human fetal osteoblast (hFOB) cells on HA-based composites, in vitro. In this work, the osteoblast cell fate process has been studied on a model hydroxyapatite-titanium (HA-Ti) system using the flow cytometry. In order to retain both HA and Ti, the novel processing technique, that is, spark plasma sintering, was suitably adopted. The cell fate processes of hFOBs, as evaluated using a flow cytometry, revealed statistically insignificant differences among HA-10 wt % Ti and HA and control (tissue culture polystyrene surface) in terms of osteoblast apoptosis, proliferation index as well as division index. For the first time, we provide quantified flow cytometry results to demonstrate that 10 wt % Ti additions to HA do not have any significant influence on the fate processes of human osteoblast-like cells, in vitro.
| Item Type: | Journal Article |
|---|---|
| Publication: | Journal of Biomedical Materials Research Part A |
| Publisher: | Wiley-Blackwell |
| Additional Information: | Copyright of this article belongs to Wiley-Blackwell. |
| Keywords: | HA-Ti; Flow Cytometry; Apoptosis; Proliferation; Cell Cycle |
| Department/Centre: | Division of Chemical Sciences > Materials Research Centre |
| Date Deposited: | 24 Sep 2013 06:26 |
| Last Modified: | 24 Sep 2013 06:26 |
| URI: | http://eprints.iisc.ac.in/id/eprint/47417 |
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