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``On-the-fly'' kinetics of enzymatic racemization using deuterium NMR in DNA-based chiral oriented media

Chan-Huot, Monique and Lesot, Philippe and Pelupessy, Philippe and Duma, Luminita and Bodenhausen, Geoffrey and Duchambon, Patricia and Toney, Michael D and Reddy, Venkateswara U and Suryaprakash, N (2013) ``On-the-fly'' kinetics of enzymatic racemization using deuterium NMR in DNA-based chiral oriented media. In: Analytical Chemistry, 85 (9). pp. 4694-4697.

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Official URL: http://dx.doi.org/10.1021/ac4004002

Abstract

We report the in situ and real-time monitoring of the interconversion of L- and D-alanine-d(3) by alanine racemase from Bacillus stearothermophilus directly observed by H-2 NMR spectroscopy in anisotropic phase. The enantiomers are distinguished by the difference of their H-2 quadrupolar splittings in a chiral liquid crystal containing short DNA fragments. The proof-of-principle, the reliability, and the robustness of this new method is demonstrated by the determination of the turnover rates of the enzyme using the Michaelis Menten model.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to American Chemical Society.
Department/Centre: Division of Chemical Sciences > NMR Research Centre (Formerly Sophisticated Instruments Facility)
Division of Chemical Sciences > Solid State & Structural Chemistry Unit
Depositing User: Francis Jayakanth
Date Deposited: 11 Jul 2013 06:38
Last Modified: 11 Jul 2013 06:38
URI: http://eprints.iisc.ac.in/id/eprint/46790

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