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Effect of lipopolysaccharide on alteration of phospholipids and their fatty acid composition in spleen and thymus by in vitro metabolic labeling

Sabarirajan, Jayaraja and Vijayaraj, Panneerselvam and Sarkar, Mary and Nachiappan, Vasanthi (2013) Effect of lipopolysaccharide on alteration of phospholipids and their fatty acid composition in spleen and thymus by in vitro metabolic labeling. In: Journal of Applied Toxicology, 33 (6). pp. 418-425.

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Official URL: http://dx.doi.org/10.1002/jat.1752

Abstract

Lipopolysaccharide (LPS) is an endotoxin, a potent stimulator of immune response and induction of LPS leads to acute lung injury (ALI)/acute respiratory distress syndrome (ARDS). ARDS is a life-threatening disease worldwide with a high mortality rate. The immunological effect of LPS with spleen and thymus is well documented; however the impact on membrane phospholipid during endotoxemia has not yet been studied. Hence we aimed to investigate the influence of LPS on spleen and thymus phospholipid and fatty acid composition by 32P]orthophosphate labeling in rats. The in vitro labeling was carried out with phosphate-free medium (saline). Time course, LPS concentration-dependent, pre- and post-labeling with LPS and fatty acid analysis of phospholipid were performed. Labeling studies showed that 50 mu g LPS specifically altered the major phospholipids, phosphatidylcholine and phosphatidylglycerol in spleen and phosphatidylcholine in thymus. Fatty acid analysis showed a marked alteration of unsaturated fatty acids/saturated fatty acids in spleen and thymus leading to immune impairment via the fatty acid remodeling pathway. Our present in vitro lipid metabolic labeling study could open up new vistas for exploring LPS-induced immune impairment in spleen and thymus, as well as the underlying mechanism.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Wiley-Blackwell.
Keywords: LPS; 32P]-Labeling; Phospholipid Metabolism; Spleen; Thymus; Fatty Acid
Department/Centre: Division of Biological Sciences > Biochemistry
Depositing User: Francis Jayakanth
Date Deposited: 11 Jun 2013 08:00
Last Modified: 12 Jun 2013 09:21
URI: http://eprints.iisc.ac.in/id/eprint/46630

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