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Circulating miRNA profile in HCV infected serum: novel insight into pathogenesis

Shwetha, Shivaprasad and Gouthamchandra, K and Chandra, Madhavi and Ravishankar, B and Khaja, MN and Das, Saumitra (2013) Circulating miRNA profile in HCV infected serum: novel insight into pathogenesis. In: SCIENTIFIC REPORTS, 3 .

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Official URL: http://dx.doi.org/10.1038/srep01555

Abstract

Changes in circulating miRNA profiles have been associated with different diseases. Here we demonstrate the circulating miRNA profile in serum of HCV infected individuals using a microRNA array that profiles the expression of 940 miRNAs. Serum samples from two HCV genotype -1 and two HCV genotype -3 infected individuals were compared with healthy controls. Expression levels of miR-134, miR-198, miR-320c and miR-483-5p that were commonly upregulated in case of both genotypes were validated in 36 individual patient serum samples. Serum miR-134, miR-320c and miR-483-5p were significantly upregulated during HCV infection. miR-320c and miR-483-5p were also upregulated in HCV-JFH1 infected cells and cell culture supernatant. Pathway analysis of putative target genes of these miRNAs indicated involvement of PI3K-Akt, NFKB and MAPK signaling pathways. Results revealed novel insights on the role of circulating miRNAs in mediating pathogenesis in HCV-infected cells.

Item Type: Journal Article
Additional Information: Copyright for this article belongs to the NATURE PUBLISHING GROUP, ENGLAND.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Depositing User: Francis Jayakanth
Date Deposited: 20 May 2013 07:06
Last Modified: 20 May 2013 07:06
URI: http://eprints.iisc.ac.in/id/eprint/46484

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