ePrints@IIScePrints@IISc Home | About | Browse | Latest Additions | Advanced Search | Contact | Help

A soluble diacylglycerol acyltransferase is involved in triacylglycerol biosynthesis in the oleaginous yeast Rhodotorula glutinis

Rani, Sapa Hima and Saha, Saikat and Rajasekharan, Ram (2013) A soluble diacylglycerol acyltransferase is involved in triacylglycerol biosynthesis in the oleaginous yeast Rhodotorula glutinis. In: MICROBIOLOGY, 159 (Part 1). pp. 155-166.

[img] PDF
microbiology_159_155_2013.pdf - Published Version
Restricted to Registered users only

Download (526kB) | Request a copy
Official URL: http://dx.doi.org/10.1099/mic.0.063156-0

Abstract

The biosynthesis of triacylglycerol (TAG) occurs in the microsomal membranes of eukaryotes. Here, we report the identification and functional characterization of diacylglycerol acyltransferase (DGAT), a member of the 10 S cytosolic TAG biosynthetic complex (TBC) in Rhodotorula glutinis. Both a full-length and an N-terminally truncated cDNA clone of a single gene were isolated from R. glutinis. The DGAT activity of the protein encoded by RgDGAT was confirmed in vivo by the heterologous expression of cDNA in a Saccharomyces cerevisiae quadruple mutant (H1246) that is defective in TAG synthesis. RgDGAT overexpression in yeast was found to be capable of acylating diacylglycerol (DAG) in an acyl-CoA-dependent manner. Quadruple mutant yeast cells exhibit growth defects in the presence of oleic acid, but wild-type yeast cells do not. In an in vivo fatty acid supplementation experiment, RgDGAT expression rescued quadruple mutant growth in an oleate-containing medium. We describe a soluble acyl-CoA-dependent DAG acyltransferase from R. glutinis that belongs to the DGAT3 class of enzymes. The study highlights the importance of an alternative TAG biosynthetic pathway in oleaginous yeasts.

Item Type: Journal Article
Additional Information: Copyrifgt for this article belongs to the Society for General Microbiology
Keywords: LYSOPHOSPHATIDIC ACID ACYLTRANSFERASE; SACCHAROMYCES- CEREVISIAE; MYCOBACTERIUM-TUBERCULOSIS; PHOSPHOLIPID-SYNTHESIS; ARABIDOPSIS-THALIANA; MOLECULAR-CLONING; ESCHERICHIA-COLI; KEY ENZYME; GENE; ACCUMULATION
Department/Centre: Division of Biological Sciences > Biochemistry
Depositing User: Francis Jayakanth
Date Deposited: 02 Apr 2013 10:16
Last Modified: 02 Apr 2013 10:16
URI: http://eprints.iisc.ac.in/id/eprint/46254

Actions (login required)

View Item View Item