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Lacrimal Proline Rich 4 (LPRR4) Protein in the Tear Fluid Is a Potential Biomarker of Dry Eye Syndrome

Aluru, Saijyothi Venkata and Agarwal, Shweta and Srinivasan, Bhaskar and Iyer, Geetha Krishnan and Rajappa, Sivakumar M and Tatu, Utpal and Padmanabhan, Prema and Subramanian, Nirmala and Narayanasamy, Angayarkanni (2012) Lacrimal Proline Rich 4 (LPRR4) Protein in the Tear Fluid Is a Potential Biomarker of Dry Eye Syndrome. In: PLOS ONE, 7 (12).

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Official URL: http://dx.doi.org/10.1371/journal.pone.0051979

Abstract

Dry eye syndrome (DES) is a complex, multifactorial, immune-associated disorder of the tear and ocular surface. DES with a high prevalence world over needs identification of potential biomarkers so as to understand not only the disease mechanism but also to identify drug targets. In this study we looked for differentially expressed proteins in tear samples of DES to arrive at characteristic biomarkers. As part of a prospective case-control study, tear specimen were collected using Schirmer strips from 129 dry eye cases and 73 age matched controls. 2D electrophoresis (2DE) and Differential gel electrophoresis (DIGE) was done to identify differentially expressed proteins. One of the differentially expressed protein in DES is lacrimal proline rich 4 protein (LPRR4). LPRR4 protein expression was quantified by enzyme immune sorbent assay (ELISA). LPRR4 was down regulated significantly in all types of dry eye cases, correlating with the disease severity as measured by clinical investigations. Further characterization of the protein is required to assess its therapeutic potential in DES.

Item Type: Journal Article
Additional Information: Copy right for this article belongs to PUBLIC LIBRARY SCIENCE,USA
Department/Centre: Division of Biological Sciences > Biochemistry
Depositing User: Francis Jayakanth
Date Deposited: 04 Feb 2013 11:39
Last Modified: 04 Feb 2013 11:39
URI: http://eprints.iisc.ac.in/id/eprint/45731

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