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Crowding, molecular volume and plasticity: An assessment involving crystallography, NMR and simulations

Selvaraj, M and Ahmad, Rais and Varshney, Umesh and Vijayan, M (2012) Crowding, molecular volume and plasticity: An assessment involving crystallography, NMR and simulations. In: JOURNAL OF BIOSCIENCES, 37 (6). pp. 953-963.

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Official URL: http://dx.doi.org/10.1007/s12038-012-9276-5

Abstract

The discrepancy between the X-ray and NMR structures of Mycobacterium tuberculosis peptidyl-tRNA hydrolase in relation to the functionally important plasticity of the molecule led to molecular dynamics simulations. The X-ray and the NMR studies along with the simulations indicated an inverse correlation between crowding and molecular volume. A detailed comparison of proteins for which X-ray and the NMR structures appears to confirm this correlation. In consonance with the reported results of the investigations in cellular compartments and aqueous solution, the comparison indicates that the crowding results in compaction of the molecule as well as change in its shape, which could specifically involve regions of the molecule important in function. Crowding could thus influence the action of proteins through modulation of the functionally important plasticity of the molecule. Selvaraj M, Ahmad R, Varshney U and Vijayan M 2012 Crowding, molecular volume and plasticity: An assessment involving crystallography, NMR and simulations. J. Biosci. 37 953-963] DOI 10.1007/s12038-012-9276-5

Item Type: Journal Article
Additional Information: Copyright for this article belongs to INDIAN ACAD SCIENCES, BANGALORE
Keywords: Molecular crowding;molecular plasticity;molecular shape;peptidyl-tRNA hydrolase;protein function
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Division of Biological Sciences > Microbiology & Cell Biology
Depositing User: Francis Jayakanth
Date Deposited: 04 Jan 2013 06:02
Last Modified: 04 Jan 2013 06:02
URI: http://eprints.iisc.ac.in/id/eprint/45621

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