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GFT projection NMR for efficient H-1/C-13 sugar spin system identification in nucleic acids

Atreya, Hanudatta S and Sathyamoorthy, Bharathwaj and Jaipuria, Garima and Beaumont, Victor and Varani, Gabriele and Szyperski, Thomas (2012) GFT projection NMR for efficient H-1/C-13 sugar spin system identification in nucleic acids. In: JOURNAL OF BIOMOLECULAR NMR, 54 (4). pp. 337-342.

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Official URL: http://dx.doi.org/10.1007/s10858-012-9687-5

Abstract

A newly implemented G-matrix Fourier transform (GFT) (4,3)D HC(C)CH experiment is presented in conjunction with (4,3)D HCCH to efficiently identify H-1/C-13 sugar spin systems in C-13 labeled nucleic acids. This experiment enables rapid collection of highly resolved relay 4D HC(C)CH spectral information, that is, shift correlations of C-13-H-1 groups separated by two carbon bonds. For RNA, (4,3)D HC(C)CH takes advantage of the comparably favorable 1'- and 3'-CH signal dispersion for complete spin system identification including 5'-CH. The (4,3)D HC(C)CH/HCCH based strategy is exemplified for the 30-nucleotide 3'-untranslated region of the pre-mRNA of human U1A protein.

Item Type: Journal Article
Additional Information: Copyright for this article belongs to SPRINGER, NETHERLANDS
Keywords: GFT projection NMR;Nucleic acids;Resonance assignment;RNA
Department/Centre: Division of Chemical Sciences > NMR Research Centre (Formerly Sophisticated Instruments Facility)
Depositing User: Francis Jayakanth
Date Deposited: 04 Jan 2013 06:00
Last Modified: 04 Jan 2013 06:00
URI: http://eprints.iisc.ac.in/id/eprint/45620

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