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H-1, C-13, N-15 assignment and secondary structure determination of glutamine amido transferase subunit of gaunosine monophosphate synthetase from Methanocaldococcus jannaschii

Ali, Rustam and Kumar, Sanjeev and Balaram, Hemalatha and Sarma, Siddhartha P (2012) H-1, C-13, N-15 assignment and secondary structure determination of glutamine amido transferase subunit of gaunosine monophosphate synthetase from Methanocaldococcus jannaschii. In: BIOMOLECULAR NMR ASSIGNMENTS, 6 (2). pp. 193-196.

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Official URL: http://dx.doi.org/10.1007/s12104-011-9354-x

Abstract

Sequence specific resonance assignments have been obtained for H-1, C-13 and N-15 nuclei of the 21 kDa (188 residues long) glutamine amido transferase subunit of guanosine monophosphate synthetase from Methanocaldococcus jannaschii. From an analysis of H-1 and C-13(alpha), C-13(beta) secondary chemical shifts, (3) JH(N)H(alpha) scalar coupling constants and sequential, short and medium range H-1-H-1 NOEs, it was deduced that the glutamine amido transferase subunit has eleven strands and five helices as the major secondary structural elements in its tertiary structure.

Item Type: Journal Article
Additional Information: Copyright for this article belongs to Springer
Keywords: Amidotransferase; Ammonia channeling; De-novo purine nucleotide biosynthesis; NMR resonance assignments; Secondary structure; Methanocaldococcus jannaschii
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Depositing User: Id for Latest eprints
Date Deposited: 07 Dec 2012 09:19
Last Modified: 11 Dec 2012 05:58
URI: http://eprints.iisc.ac.in/id/eprint/45226

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