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High resolution methyl selective C-13-NMR of proteins in solution and solid state

Jaipuria, Garima and Lobo, Nitin Prakash and Shet, Divya and Atreya, Hanudatta S (2012) High resolution methyl selective C-13-NMR of proteins in solution and solid state. In: Journal of Biomolecular NMR, 54 (1). pp. 33-42.

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Official URL: http://dx.doi.org/10.1007/s10858-012-9647-0


New C-13-detected NMR experiments have been devised for molecules in solution and solid state, which provide chemical shift correlations of methyl groups with high resolution, selectivity and sensitivity. The experiments achieve selective methyl detection by exploiting the one bond J-coupling between the C-13-methyl nucleus and its directly attached C-13 spin in a molecule. In proteins such correlations edit the C-13-resonances of different methyl containing residues into distinct spectral regions yielding a high resolution spectrum. This has a range of applications as exemplified for different systems such as large proteins, intrinsically disordered polypeptides and proteins with a paramagnetic centre.

Item Type: Journal Article
Publication: Journal of Biomolecular NMR
Publisher: Springer
Additional Information: Copyright for this article is belongs to Springer.
Keywords: Methyl groups;Methyl COSY;Methyl selective NMR;C-13 direct detection;Solid state NMR;Paramagnetic proteins
Department/Centre: Division of Chemical Sciences > NMR Research Centre (Formerly Sophisticated Instruments Facility)
Division of Chemical Sciences > Solid State & Structural Chemistry Unit
Division of Physical & Mathematical Sciences > Physics
Date Deposited: 22 Nov 2012 10:33
Last Modified: 22 Nov 2012 10:33
URI: http://eprints.iisc.ac.in/id/eprint/45136

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