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Reduced dimensionality 3D HNCAN for unambiguous HN, CA and N assignment in proteins

Rout, Manoj Kumar and Mishra, Pushpa and Atreya, Hanudatta S and Hosur, Ramakrishna V (2012) Reduced dimensionality 3D HNCAN for unambiguous HN, CA and N assignment in proteins. In: Journal of Magnetic Resonance, 216 . pp. 161-168.

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Official URL: http://dx.doi.org/10.1016/j.jmr.2012.01.022


We present here an improvisation of HNN (Panchal, Bhavesh et al., 2001) called RD 3D HNCAN for backbone (HN, CA and N-15) assignment in both folded and unfolded proteins. This is a reduced dimensionality experiment which employs CA chemical shifts to improve dispersion. Distinct positive and negative peak patterns of various triplet segments along the polypeptide chain observed in HNN are retained and these provide start and check points for the sequential walk. Because of co-incrementing of CA and N-15, peaks along one of the dimensions appear at sums and differences of the CA and N-15 chemical shifts. This changes the backbone assignment protocol slightly and we present this in explicit detail. The performance of the experiment has been demonstrated using Ubiquitin and Plasmodium falciparum P2 proteins. The experiment is particularly valuable when two neighboring amino acid residues have nearly identical backbone N-15 chemical shifts. (C) 2012 Elsevier Inc. All rights reserved.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Elsevier Science.
Keywords: Reduced dimensionality;Triplet peak pattern;Backbone assignment;Check points;HNN;NMR
Department/Centre: Division of Chemical Sciences > NMR Research Centre (Formerly Sophisticated Instruments Facility)
Depositing User: Id for Latest eprints
Date Deposited: 12 May 2012 10:45
Last Modified: 12 May 2012 10:47
URI: http://eprints.iisc.ac.in/id/eprint/44402

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