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Efficient sequential assignments in proteins with reduced dimensionality 3D HN(CA)NH

Chandra, Kousik and Jaipuria, Garima and Shet, Divya and Atreya, Hanudatta S (2012) Efficient sequential assignments in proteins with reduced dimensionality 3D HN(CA)NH. In: Journal of Biomolecular NMR, 52 (2). pp. 115-126.

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We present reduced dimensionality (RD) 3D HN(CA)NH for efficient sequential assignment in proteins. The experiment correlates the N-15 and H-1 chemical shift of a residue ('i') with those of its immediate N-terminal (i - 1) and C-terminal (i + 1) neighbors and provides four-dimensional chemical shift correlations rapidly with high resolution. An assignment strategy is presented which combines the correlations observed in this experiment with amino acid type information obtained from 3D CBCA(CO)NH. By classifying the 20 amino acid types into seven distinct categories based on C-13(beta) chemical shifts, it is observed that a stretch of five sequentially connected residues is sufficient to map uniquely on to the polypeptide for sequence specific resonance assignments. This method is exemplified by application to three different systems: maltose binding protein (42 kDa), intrinsically disordered domain of insulin-like growth factor binding protein-2 and Ubiquitin. Fast data acquisition is demonstrated using longitudinal H-1 relaxation optimization. Overall, 3D HN(CA)NH is a powerful tool for high throughput resonance assignment, in particular for unfolded or intrinsically disordered polypeptides.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Springer.
Keywords: Sequence specific resonance assignment;Reduced dimensionality NMR;Protein structure;GFT NMR
Department/Centre: Division of Chemical Sciences > NMR Research Centre (Formerly Sophisticated Instruments Facility)
Depositing User: Id for Latest eprints
Date Deposited: 04 Apr 2012 12:17
Last Modified: 04 Apr 2012 12:17
URI: http://eprints.iisc.ac.in/id/eprint/44171

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