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Interplay between NS3 protease and human La protein regulates translation-replication switch of Hepatitis C virus

Ray, Upasana and Das, Saumitra (2011) Interplay between NS3 protease and human La protein regulates translation-replication switch of Hepatitis C virus. In: SCIENTIFIC REPORTS, 1 .

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Official URL: http://www.nature.com/srep/2011/110614/srep00001/f...

Abstract

HCV NS3 protein plays a central role in viral polyprotein processing and RNA replication. We demonstrate that the NS3 protease (NS3(pro)) domain alone can specifically bind to HCV-IRES RNA, predominantly in the SLIV region. The cleavage activity of the NS3 protease domain is reduced upon HCV-RNA binding. More importantly, NS3(pro) binding to the SLIV hinders the interaction of La protein, a cellular IRES-trans acting factor required for HCV IRES-mediated translation, resulting in inhibition of HCV-IRES activity. Although overexpression of both NS3(pro) as well as the full length NS3 protein decreased the level of HCV IRES mediated translation, replication of HCV replicon RNA was enhanced significantly. These observations suggest that the NS3(pro) binding to HCV IRES reduces translation in favor of RNA replication. The competition between the host factor (La) and the viral protein (NS3) for binding to HCV IRES might regulate the molecular switch from translation to replication of HCV.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Nature Publishing Group.
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Depositing User: Id for Latest eprints
Date Deposited: 17 Nov 2011 09:33
Last Modified: 17 Nov 2011 09:33
URI: http://eprints.iisc.ac.in/id/eprint/42103

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