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Preferential condensation of SAR-DNA by histone H1 and its SPKK containing octapeptide repeat motif

Khadake, Jyoti R and Rao, MRS (1997) Preferential condensation of SAR-DNA by histone H1 and its SPKK containing octapeptide repeat motif. In: FEBS Letters, 400 (2). pp. 193-196.

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Official URL: http://dx.doi.org/10.1016/S0014-5793(96)01393-2

Abstract

Linker histone H1 binds preferentially the scaffold associated region (SAR) DNA elements that contain characteristic oligo dA . dT tracts. In the present study, we have compared the condensation brought about by histone H1 of a SAR DNA fragment in the histone spacer region of Drosophila melanogaster with that of a random DNA (pBR322 EcoRI-SalI) fragment by circular dichroism spectroscopy. The condensation of the SAR DNA fragment by histone H1 is 3-4-fold higher than that of the random DNA fragment. A 16-mer peptide, ATPKKSTKKTPKKAKK, the sequence that is present in the C-terminus of histone H1d, which has recently been shown to possess DIVA and chromatin condensing properties, also condenses the SAR DNA fragment preferentially in a highly cooperative manner. We have proposed a model for the dynamics of chromatin structure involving histone H1-SAR DNA interaction through SPKK containing peptide motifs and its competition by AT-hook peptides present in the nonhistone chromosomal proteins like HMG-I and HMG-Y.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Elsevier Science.
Keywords: Scaffold associated region;Histone H1;AT-hook peptide motif
Department/Centre: Division of Biological Sciences > Biochemistry
Depositing User: Ms V Mangala
Date Deposited: 30 Jun 2011 14:06
Last Modified: 30 Jun 2011 14:06
URI: http://eprints.iisc.ac.in/id/eprint/38364

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