7Li and 23Na NMR studies of transmembrane cation transport mediated by ionophore lasalocid A

Juvvadi, Padmaja and Kalapat, Easwaran (1998) 7Li and 23Na NMR studies of transmembrane cation transport mediated by ionophore lasalocid A. In: Journal of Peptide Science, 4 (1). pp. 15-20.

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Abstract

Ion transport across phospholipid vesicles was studied by 7Li and 23Na-NMR using an aqueous anionic paramagnetic shift reagent, dysprosium nitrilotriacetate [Dy(NTA)2]3?, mediated by ionophores, lasalocid A and A23187. The intra- and extracellular 7Li and 23Na-NMR signals were well separated (20?Hz) at mM concentration of the shift reagent. The observed data on the rate constant for lithium transport across DPPC vesicles at various concentrations of the ionophores indicated that lasalocid A is a more efficient carrier for lithium ion compared with the sodium ion transport by this ionophore, while A23187 was not specific to either of the ions (Li or Na). ©1998 European Peptide Society and John Wiley & Sons, Ltd.

Item Type:	Journal Article
Publication:	Journal of Peptide Science
Publisher:	John Wiley and Sons
Additional Information:	Copyright of this article belongs to John Wiley and Sons.
Keywords:	Ion transport;kinetics;lithium;NMR;shift reagent;vesicles.
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	09 Jun 2011 06:53
Last Modified:	09 Jun 2011 06:53
URI:	http://eprints.iisc.ac.in/id/eprint/38164

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