Cloning, expression, purification, crystallization and preliminary X-ray studies of the mannose-binding lectin domain of MSMEG_3662 from Mycobacterium smegmatis

Patra, Dhabaleswar and Sharma, Alok and Chandran, Divya and Vijayan, Mamannamana (2011) Cloning, expression, purification, crystallization and preliminary X-ray studies of the mannose-binding lectin domain of MSMEG_3662 from Mycobacterium smegmatis. In: Acta Crystallographica Section F, 67 (Part 5). pp. 596-599.

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Abstract

The mannose-binding lectin domain of MSMEG_3662 from Mycobacterium smegmatis has been cloned, expressed, purified and crystallized and the crystals have been characterized using X-ray diffraction. The Matthews coefficient suggests the possibility of two lectin domains in the triclinic cell. The amino-acid sequence of the domain indicates structural similarity to well characterized beta-prism II fold lectins.

Item Type:	Journal Article
Publication:	Acta Crystallographica Section F
Publisher:	John Wiley & Sons
Additional Information:	Copyright of this article belongs to John Wiley & Sons.
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	17 Jun 2011 08:19
Last Modified:	17 Jun 2011 08:19
URI:	http://eprints.iisc.ac.in/id/eprint/37815

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