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Hormonal modulation of secretion of immunoreactive riboflavin carrier protein by adult rat Leydig cells in vitro

Subramanian, S and Adiga, PR (1996) Hormonal modulation of secretion of immunoreactive riboflavin carrier protein by adult rat Leydig cells in vitro. In: Indian Journal of Biochemistry & Biophysics, 33 (4). pp. 274-280.

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Abstract

Adult rat Leydig cells in culture synthesize and secrete riboflavin carrier protein (RCP) as demonstrated by [S-35]-methionine incorporation into newly synthesized proteins followed by immunoprecipitation as well as specific radioimmunoassay. LH stimulates the secretion of RCP 4-fold which could be inhibited upto 75% by an aromatase inhibitor. 8-bromo-cyclic AMP and cholera toxin could mimic the LH stimulated secretion of the carrier protein. The extent of stimulation of RCP secretion brought about by exogenous estradiol-17 beta is comparable to that of LH. The antiestrogen tamoxifen, when added along with either LH or estrogen, inhibited the stimulated levels significantly. These results show that the estrogen-inducible riboflavin carrier is secreted by Leydig cells under positive regulation of LH.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to National institute of science communication and information resources.
Department/Centre: Division of Biological Sciences > Biochemistry
Depositing User: Ms V Mangala
Date Deposited: 28 Apr 2011 09:54
Last Modified: 23 Oct 2018 10:06
URI: http://eprints.iisc.ac.in/id/eprint/37286

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