Effect of fenvalerate, a pyrethroid insecticide on membrane fluidity

Sarkar, Saumendra N and Balasubramanian, SV and Sikdar, SK (1993) Effect of fenvalerate, a pyrethroid insecticide on membrane fluidity. In: Biochimica et Biophysica Acta (BBA) - Biomembranes, 1147 (1). pp. 137-142.

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Abstract

Fenvalerate is a commonly used pyrethroid insecticide, used to control a wide range of pests. We have studied its interaction with the membrane using fluorescence polarization and differential scanning calorimetry (DSC) techniques. Fenvalerate was found to decrease the DPH fluorescence polarization value of synaptosomal and microsomal membrane, implicating that it makes the membrane more fluid. At different concentrations of fenvalerate, the activation energy of the probe molecule in the membrane also changes revealed from the change in slope of the Arrhenius plot. At higher concentrations the insecticide slowly saturates the membrane. The effects of fenvalerate on model membrane were also studied with liposomes reconstituted with dipalmitoylphosphatidylcholine (DPPC). Fenvalerate decreased the phase transition temperature (Tm) of DPPC by 1.5 °C at 40 μM concentration, but there was no effect on the cooperativity of the transition as interpreted from the DSC thermogram. From the change in the thermogram profile with fenvalerate it has been interpreted that it localizes in the acyl chain region of the lipid, possibly between C10 and C16 region and weakens the acyl chain packing. Fenvalerate was also found to interact with DPPC liposomes containing cholesterol to fluidize it.

Item Type:	Journal Article
Publication:	Biochimica et Biophysica Acta (BBA) - Biomembranes
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Keywords:	Fenvalerate;Insecticide;Fluorescence polarization;DSC; Membrane fluidity.
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	08 Feb 2011 06:42
Last Modified:	08 Feb 2011 06:42
URI:	http://eprints.iisc.ac.in/id/eprint/35432

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