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Reinstate hydrogen peroxide as the product of alternative oxidase of plant mitochondria

Bhate, Radha and Ramasarma, T (2010) Reinstate hydrogen peroxide as the product of alternative oxidase of plant mitochondria. In: Indian Journal of Biochemistry & Biophysics, 47 (5). pp. 306-310.

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Official URL: http://nopr.niscair.res.in/handle/123456789/10525


Chill treatment of potato tubers for 8 days induced mitochondrial O-2 consumption by cyanide-insensitive alternative oxidase (AOX). About half of the total O-2 consumption in such mitochondria was found to be sensitive to salicylhydroxamate (SHAM), a known inhibitor of AOX activity. Addition of catalase to the reaction mixture of AOX during the reaction decreased the rate of SHAM-sensitive O-2 consumption by nearly half, and addition at the end of the reaction released half of the O-2 consumed by AOX, both typical of catalase action on H2O2. This reaffirmed that the product of reduction of O-2 by plant AOX was H2O2 as found earlier and not H2O as reported in some recent reviews.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to National Institute of Science Communication and Information Resources.
Keywords: Mitochondrial alternative oxidase; Chilling treatment; Potato; SHAM-sensitive activity; Catalase-dependent O-2 release; H2O2
Department/Centre: Division of Biological Sciences > Biochemistry
Depositing User: Id for Latest eprints
Date Deposited: 03 Feb 2011 05:52
Last Modified: 11 Oct 2018 14:27
URI: http://eprints.iisc.ac.in/id/eprint/35236

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