Isolation and characterization of a cytokinin-binding protein from cucumber cotyledons

Jayabaskaran, Chellathai (1990) Isolation and characterization of a cytokinin-binding protein from cucumber cotyledons. In: Plant Growth Regulation, 9 (1). pp. 9-17.

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Abstract

A protein which binds specifically to [3H]-zeatin has been isolated from cucumber cotyledons by chromatographic techniques. Its binding to [3H]-zeatin was inhibited remarkably by the addition of non-radioactive cytokinins and the order of inhibition was zeatin > -zeatin riboside > N6-(Delta2-isopentenyl)adenine > N6-(Delta2-isopentenyl)adenosine > N6-benzyl-adenosine > kinetin riboside. This protein behaved as a soluble protein with an apparent molecular size of 43,000 daltons on gel filtration through calibrated Sephadex G-100 column. The dissociation constant, Kd, of the protein-zeatin complex was about 4 × 10–7 M.

Item Type:	Journal Article
Publication:	Plant Growth Regulation
Publisher:	Springer
Additional Information:	Copyright of this article belongs to Springer.
Keywords:	binding protein;cytokinins and specificity
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	27 Apr 2011 04:35
Last Modified:	27 Apr 2011 04:35
URI:	http://eprints.iisc.ac.in/id/eprint/35053

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