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Control of morphogenesis in tobacco protoplast cultures: organogenesis vs embryogenesis

Rao, KS and Gunasekari, K (1991) Control of morphogenesis in tobacco protoplast cultures: organogenesis vs embryogenesis. In: Indian Journal of Biochemistry & Biophysics, 28 (5-6). pp. 467-471.

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Official URL: http://www.ncbi.nlm.nih.gov/pubmed/1812084


The morphogenetic pathway leading to plant differentiation in tobacco mesophyll protoplasts could be regulated. The course of development via organogenesis or embryogenesis was controlled by manipulating nutrient media, culture conditions and hormone requirements. A lowering of molarity of medium after 5 weeks of protoplast culture, inclusion of GA3 (0.5 mg/l) in the medium for first 8 weeks of culture and exclusion of reduced nitrogen in the medium resulted in shoot organogenesis, while maintenance of higher molarity of the medium till 8 weeks, reduced nitrogen in the medium and removal of 2, 4-D after 5 weeks of culture induced embryogenesis. Regenerability of viable plants was obtained by both developmental pathways. The implications of tobacco embryogenesis system in plant molecular genetics were highlighted.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to National Institute of Science Communication and Information Resources.
Department/Centre: Division of Biological Sciences > Biochemistry
Depositing User: Ms Mary Ranjeetha
Date Deposited: 10 Nov 2010 05:11
Last Modified: 10 Nov 2010 05:11
URI: http://eprints.iisc.ac.in/id/eprint/33716

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