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Isolation Of A Cytokinin-Binding Protein From Cucumis-Sativus Cotyledons

Jayabaskaran, C (1988) Isolation Of A Cytokinin-Binding Protein From Cucumis-Sativus Cotyledons. In: Proceedings of the Indian Academy of Sciences - Section B, 98 (4). pp. 269-276.

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A cytokinin-binding protein which exists as monomer and dimer was isolated from cucumber (Cucumis sativus L. var Guntur) cotyledons by affinity chromatography on AH-Sepharose-pi6 Ap /s> column. The protein bound to [3H]-N6-(Δ2-isopentenyl) adenosine trialcohol. On Sephadex G-50 chromatography it gave 2 peaks corresponding to molecular weight 4000 and 8000 daltons. On sodium dodecyl sulphate-polyacrylamide gel electrophoresis, it gave only one band with an apparent molecular weight of 4000 daltons.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Indian Academy of Sciences.
Keywords: Cucumis sativus;cytokinin;binding protein;N6-(Δ2-isopentenyl) adenosine;affinity chromatography.
Department/Centre: Division of Biological Sciences > Biochemistry
Depositing User: Ms Mary Ranjeetha
Date Deposited: 24 Sep 2010 09:55
Last Modified: 24 Sep 2010 09:55
URI: http://eprints.iisc.ac.in/id/eprint/32341

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