Metal binding to pyridoxal derivatives. An NMR study of the interaction of Eu(III) with pyridoxal phosphate and pyridoxamine phosphate

Rao, Narasinga BN and Ramakrishnan, C and Balaram, P (1979) Metal binding to pyridoxal derivatives. An NMR study of the interaction of Eu(III) with pyridoxal phosphate and pyridoxamine phosphate. In: Journal of Biosciences, 1 (1). pp. 35-47.

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Abstract

The solution conformations of pyridoxal-5’-phosphate and pyridoxamine-5’-phosphate have been investigated using Eu(III) as a nuclear magnetic resonance shift probe. Binding of Eu(III) to pyridoxal phosphate results in the formation of two complexes, at the phosphate group and the \alpha-hydroxy-aldehyde moiety, which are in slow exchange on the nuclear magnetic resonance time-scale. The lanthanide-induced pseudo contact shifts calculated using the McConnell-Robertson equation. (J. Chem. Soc. (1950), 22, 1561) are in good agreement with the experimentally observed values for both pyridoxal phosphate and pyridoxamine phosphate and lead to a family of closely related conformations.

Item Type:	Journal Article
Publication:	Journal of Biosciences
Publisher:	Indian Academy of Sciences
Additional Information:	Copyright for this article belongs to Indian Academy of Sciences.
Keywords:	conformation;lanthanide;nuclear magnetic resonance;pyridoxal-5’-phosphate;pyridoxamine-5’-phosphate
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	12 May 2005
Last Modified:	19 Sep 2010 04:18
URI:	http://eprints.iisc.ac.in/id/eprint/3182

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