Trishanku, a novel regulator of cell-type stability and morphogenesis in Dictyostelium discoideum

Jaiswal, Jyoti K and Mujumdar, Nameeta and MacWilliams, Harry K and Nanjundiah, Vidyanand (2006) Trishanku, a novel regulator of cell-type stability and morphogenesis in Dictyostelium discoideum. In: Differentiation, 74 (9-10). pp. 596-607.

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Abstract

We have identified a novel gene, trishanku (triA), by random insertional mutagenesis of Dictyostelium discoideum. TriA is a Broad complex Tramtrack bric-a-brac domain-containing protein that is expressed strongly during the late G2 phase of cell cycle and in presumptive spore (prespore (psp)) cells. Disrupting triA destabilizes cell fate and reduces aggregate size; the fruiting body has a thick stalk, a lowered spore: stalk ratio, a sub-terminal spore mass and small, rounded spores. These changes revert when the wild-type triA gene is re-expressed under a constitutive or a psp-specific promoter. By using short- and long-lived reporter proteins, we show that in triA(-) slugs the prestalk (pst)/psp proportion is normal, but that there is inappropriate transdifferentiation between the two cell types. During culmination, regardless of their current fate, all cells with a history of pst gene expression contribute to the stalk, which could account for the altered cell-type proportion in the mutant.

Item Type:	Journal Article
Publication:	Differentiation
Publisher:	International Society of Differentiation
Additional Information:	Copyright of this article belongs to International Society of Differentiation.
Keywords:	REMI;differentiation;pattern formation;BTB domain;trishanku; beta glucoronidase;labile beta galactosidase.
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited:	01 Sep 2010 08:36
Last Modified:	19 Sep 2010 06:12
URI:	http://eprints.iisc.ac.in/id/eprint/30719

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