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Conformational Effects on Peptide Aggregation in Organic Solvents: Spectroscopic Studies of Two Chemotactic Tripeptide Analogs

Raj, Antony P and Balaram, P (1985) Conformational Effects on Peptide Aggregation in Organic Solvents: Spectroscopic Studies of Two Chemotactic Tripeptide Analogs. In: Biopolymers, 24 (7). pp. 1131-1146.

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Abstract

The aggregation behavior of the chemotactic peptide analogs, Formyl-Met-Leu-Phe-OMe (1) and Formyl-Met-AibPhe-OMe ${(2)}_1$, has been studied in chloroform and dimethylsulfoxide over the concentration range of 0.2-110 mM by 'H-nmr spectroscopy. Both peptides associate in $CD{Cl}_3$, at concentrations \geq 2 mM, while there is no evidence for aggregation in ${({CD}_3)}_2SO$. Analog 1 adopts an extended conformation in both solvents favoring association to form \beta-sheet structures. A folded, \gamma-turn conformation involving a 3 \rightarrow 1 hydrogen bond between Met CO and Phe NH is supported by 1H-, 13Gnmr, and ir studies of analog 2. The influence of backbone conformation on the ease of peptide aggregation is demonstrated by ir studies in $CH{Cl}_3$ and CD studies in dioxane.

Item Type: Journal Article Biopolymers John Wiley & Sons, Inc Copyright for this article belongs to John Wiley & Sons, Inc. Division of Biological Sciences > Molecular Biophysics Unit 07 Mar 2005 19 Sep 2010 04:18 http://eprints.iisc.ac.in/id/eprint/2875

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