Rao, KS and Seshadri Sastry, P (1961) Fatty acid component of vitamin A ester in sheep liver. In: Archives of Biochemistry and Biophysics, 95 (2). pp. 285-289.
PDF
Sheep.pdf - Published Version Restricted to Registered users only Download (421kB) | Request a copy |
Abstract
Vitamin A, when extracted along with other lipids from sheep liver, had an E1cm.1% value of 14.4, which was raised to 45.57 on removal of the phospholipids by cold acetone. Selective hydrolysis of triglycerides by an extract of acetone-dried sheep pancreas in the presence of HgCl2 as inhibitor of vitamin A esterase, followed by chromatography through alumina gave a product with E1cm.1% value of 276. This on chromatography through magnesium oxide raised the E1cm.1, value to 601.5, representing 64% pure vitamin A ester calculated as palmitate, and the total recovery was 23% of the starting oil. The purified ester preparation, when subjected to reverse-phase chromatography on silicone-impregnated paper, gave a single ultraviolet fluorescent band. The fluorescent band on hydrolysis gave only one fatty acid. This was conclusively identified to be palmitic acid.
Item Type: | Journal Article |
---|---|
Publication: | Archives of Biochemistry and Biophysics |
Publisher: | Elsevier Science |
Additional Information: | Copyright of this article belongs to Elsevier Science. |
Department/Centre: | Division of Biological Sciences > Biochemistry |
Date Deposited: | 16 Jun 2010 07:35 |
Last Modified: | 19 Sep 2010 06:09 |
URI: | http://eprints.iisc.ac.in/id/eprint/28451 |
Actions (login required)
View Item |