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Studies on the structure of the chromosomes - I. Allium cepa-Stain fixatives and their utility

Subramanyam, S and Subramaniam, MK (1962) Studies on the structure of the chromosomes - I. Allium cepa-Stain fixatives and their utility. In: Proceedings of the Indian Academy of Sciences - Section B, 55 (5). pp. 276-284.

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Official URL: http://www.springerlink.com/content/w8735351283lx8...


The structure of the mitotic chromosomes of Allium cepa has been elucidated by controlling the temperature and time of exposure of fresh roots to stain fixatives. The details seen in material stained in N HCl-orcein for 8 min. at 60° C. and squashed after varying intervals of storage at room temperature were essentially similar to pictures obtained with 1% aceto-orcein and 1% aceto-orcein-N HCl (10:1) under identical conditions of handling. The chromosomes appear quadri-partite at metaphase and bi-partite at anaphase. A rare instance of the precocious assumption of a quadri-partite condition by two anaphase chromosomes is illustrated. Caduceus coiling of chromonemata was seen in chromosome bridges also. Chromosomes have material easily dissociable from the chromonemata and their removal does not affect the structural integrity of the chromosome.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Springer.
Department/Centre: Division of Biological Sciences > Biochemistry
Depositing User: Ms TV Yashodha
Date Deposited: 14 Jun 2010 11:44
Last Modified: 14 Jun 2010 11:44
URI: http://eprints.iisc.ac.in/id/eprint/28387

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