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Action of N-bromosuccinimide on sperm whale myoglobin

Rao, GJS and Cama, HR (1964) Action of N-bromosuccinimide on sperm whale myoglobin. In: Biochimica et Biophysica Acta (BBA) - General Subjects, 86 (2). pp. 309-316.

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Official URL: http://dx.doi.org/10.1016/0304-4165(64)90057-1

Abstract

Previously, it was reported from this laboratory that the heme groups of hemoglobin are “buried” within globin at pH 4.0 and not dissociated, on the basis of the obiligatory requirement of urea for the reaction of N-bromosuccinimide with the heme groups of hemoglobin at pH4.0, and also on the basis of the “normalization” of the spectrum of hemoglobin at this pH in the presence of urea or sucrose. In the present study, it has been shown that the behaviour of sperm whale myoglobin with respect to its reaction with N-bromosuccinimide and with respect to spectral “normalization” in urea or sucrose are essentially similar to that of hemoglobin. It has also been demonstrated that the spectral “normalization” obtained with crystalline hemin is not identical with that obtained with either hemoglobin or myoglobin. The bearing of the results of the present study on the earlier work on hemoglobin is indicated.

Item Type: Journal Article
Additional Information: Copyright of this article belongs to Elsevier Science.
Department/Centre: Division of Biological Sciences > Biochemistry
Depositing User: Ms TV Yashodha
Date Deposited: 21 May 2010 11:17
Last Modified: 19 Sep 2010 06:07
URI: http://eprints.iisc.ac.in/id/eprint/28011

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