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Collagen IV-Mediated Signalling is Involved in Progenitor Leydig Cell Proliferation

Anbalagan, M and Rao, AJ (2004) Collagen IV-Mediated Signalling is Involved in Progenitor Leydig Cell Proliferation. In: Reproductive Biomedicine Online, 9 (4). pp. 391-403.

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Official URL: http://dx.doi.org/10.1016/S1472-6483(10)61274-6


In rats, during postnatal Leydig cell development, the progenitor Leydig cells (PLC) proliferate actively during days 14-21 of postnatal life. Luteinizing hormone (LH) is known to stimulate Leydig cell proliferation and oestradiol 17\beta inhibits this process. In order to identify the molecules involved in Leydig cell proliferation, differentially expressed genes in proliferating and non-proliferating PLC isolated from vehicle and oestradiol 17\beta treated rats respectively, were analysed by differential display reverse transcription polymerase chain reaction (DD-RT-PCR). Results revealed that the expression of collagen IV \alpha (Col IV \alpha ), a subunit of extracellular matrix (ECM) protein collagen IV, was down regulated in PLC isolated from oestradiol 17\beta treated rats. Studies on stage specific expression of Col IV \alpha during Leydig cell development revealed that this transcript is abundantly expressed at the stage where Leydig cell proliferation is maximal and the expression of this transcript decreased during differentiation of Leydig cells, which is associated with loss of proliferation. These observations suggest that Col IV \alpha is important for PLC proliferation. Stimulation of PLC proliferation in vitro in the presence collagen IV provides additional support for the conclusion that collagen IV mediated signalling is involved in PLC proliferation. Further studies revealed that active forms of focal adhesion kinase (FAK) and mitogen activated protein kinase 1/2 (MAPK 1/2), the intracellular signalling molecules that are known to mediate ECM protein signalling are present only in proliferating forms of Leydig cells and are absent in non-proliferating Leydig cells. These results suggest that collagen IV mediated signalling is involved in PLC proliferation.

Item Type: Journal Article
Publication: Reproductive Biomedicine Online
Publisher: Reproductive Healthcare Limited
Additional Information: The copyright for this article belongs to Reproductive Healthcare Limited.
Keywords: collagen IV;FAK;MAPK1/2;oestradiol 17 beta;progenitor Leydig cell
Department/Centre: Division of Biological Sciences > Biochemistry
Date Deposited: 18 Feb 2005
Last Modified: 19 Jan 2012 09:23
URI: http://eprints.iisc.ac.in/id/eprint/2796

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