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Study of thermal fluctuations during thermal denaturation of DNA

Nagapriya, KS and Raychaudhuri, AK and Chatterji, D (2005) Study of thermal fluctuations during thermal denaturation of DNA. In: Conference on Fluctuations and Noise in Biological, Biophysical, and Biomedical Systems III, MAY 24-26, 2005, Austin.

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In this paper we address the fundamental issue of temperature fluctuation during the thermal denaturation (or the unzipping of the two strands on heating) of double stranded (ds) DNA. From our experiments we observe the presence of extremely high thermal fluctuations during DNA denaturation. This thermal fluctuation is several orders higher than the thermal fluctuation at temperatures away from the denaturation temperature range. This fluctuation is absent in single stranded (ss) DNA. The magnitude of fluctuation is much higher in heteropolymeric DNA and is almost absent in short homopolymeric DNA fragments. The temperature range over which the denaturation occurs (i.e., over which the thermal fluctuation is large) depends on the length of the DNA and is largest for the longest DNA.

Item Type: Conference Paper
Additional Information: Copyright of this article belongs to The International Society for Optical Engineering.
Department/Centre: Division of Physical & Mathematical Sciences > Physics
Depositing User: Ms G Yashodha
Date Deposited: 04 Jun 2010 10:24
Last Modified: 04 Jun 2010 10:24
URI: http://eprints.iisc.ac.in/id/eprint/27541

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