Studies on the tryptophan residues of soybean agglutinin. Involvement in saccharide binding

Swamy, Musti Joginadha and Surolia, Avadhesha (1989) Studies on the tryptophan residues of soybean agglutinin. Involvement in saccharide binding. In: Bioscience Reports, 9 (2). pp. 189-198.

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Abstract

Modification of tryptophan side chains of soybean agglutinin (SBA) with N-bromosuccinimide results in a loss of the hemagglutinating and carbohydrate binding activities of the protein. One residue/subunit is probably essential for the binding activity. Modification leads to a large decrease in the fluorescene of the protein accompained by a blue shift. Iodide ion quenching of the protein fluorescence shows that saccharide binding results in a decreased accessibility of some of the tryptophan side chains. These results strongly point towards the involvement of tryptophan residues in the active site of SBA.

Item Type:	Journal Article
Publication:	Bioscience Reports
Publisher:	Springer
Additional Information:	Copy right of this article belongs to Springer.
Keywords:	saccharide binding -soybean agglutinin -tryptophan.
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	03 Jun 2010 06:47
Last Modified:	19 Sep 2010 06:01
URI:	http://eprints.iisc.ac.in/id/eprint/27532

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