Preliminary X-ray crystallographic analysis of 2-methylcitrate synthase from Salmonella typhimurium

Chittori, Sagar and Simanshu, DK and Savithri, HS and Murthy, MRN (2010) Preliminary X-ray crystallographic analysis of 2-methylcitrate synthase from Salmonella typhimurium. In: Acta Crystallographica Section F, 66 (Part 4). pp. 467-470.

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Abstract

Analysis of the genomic sequences of Escherichia coli and Salmonella typhimurium has revealed the presence of several homologues of the well studied citrate synthase (CS). One of these homologues has been shown to code for 2-methylcitrate synthase (2-MCS) activity. 2-MCS catalyzes one of the steps in the 2-methylcitric acid cycle found in these organisms for the degradation of propionate to pyruvate and succinate. In the present work, the gene coding for 2-MCS from S. typhimurium (StPrpC) was cloned in pRSET-C vector and overexpressed in E. coli. The protein was purified to homogeneity using Ni-NTA affinity chromatography. The purified protein was crystallized using the microbatch-under-oil method. The StPrpC crystals diffracted X-rays to 2.4 A resolution and belonged to the triclinic space group P1, with unit-cell parameters a = 92.068, b = 118.159, c = 120.659 A, alpha = 60.84, beta = 67.77, gamma = 81.92 degrees. Computation of rotation functions using the X-ray diffraction data shows that the protein is likely to be a decamer of identical subunits, unlike CSs, which are dimers or hexamers.

Item Type:	Journal Article
Publication:	Acta Crystallographica Section F
Publisher:	John Wiley and Sons
Additional Information:	Copyright of this article belongs to John Wiley and Sons.
Keywords:	Salmonella typhimurium; propionate metabolism; 2-methylcitric acid cycle; 2-methylcitrate synthase; citrate synthase; rotation function
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit Division of Biological Sciences > Biochemistry
Date Deposited:	08 Jun 2010 06:42
Last Modified:	19 Sep 2010 06:00
URI:	http://eprints.iisc.ac.in/id/eprint/27269

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