Structural studies of the hen's egg lipovitellins. NMR and fluorescence investigations

Rao, DN Ramakrishna and Mahadevan, S and Balaram, P (1978) Structural studies of the hen's egg lipovitellins. NMR and fluorescence investigations. In: Chemistry and Physics of Lipids, 22 (2). pp. 105-113.

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Abstract

The pH dependent reversible association-dissociation reaction of α- and β-lipovitellins from egg yolk has been studied by 1H NMR and fluorescence probe methods. Increased mobility of the choline methyl groups has been demonstrated on dissociation. The lipid methylene resonance of β-lipovitellin shows clear doublet character suggesting that the fatty acid chains exist in distinct environments. The high field component increases with temperature but is suppressed on treatment with pronase, suggesting a significant role for proteins in maintaining the differences in lipid environments. 1-Anilino-8-naphthalene sulfonate has been shown to bind less effectively to the monomeric lipovitellins. This is in agreement with earlier results suggesting that dissociation may be accompanied by increased hydration and conformational changes.

Item Type:	Journal Article
Publication:	Chemistry and Physics of Lipids
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	20 May 2010 06:23
Last Modified:	19 Sep 2010 06:00
URI:	http://eprints.iisc.ac.in/id/eprint/27164

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