Imprinting and Transcriptional Regulation Mechanism of the SLC22A18 gene from human chromosome segment 11p15.5

Ali, Mahmood and Kumar, Arun (2004) Imprinting and Transcriptional Regulation Mechanism of the SLC22A18 gene from human chromosome segment 11p15.5. In: 28th All India Cell Biology Conference and Symposium on Genome Biology, Dec 1-3, 2004, Chandigarh, India, p. 70.

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Abstract

Aim: To understand the regulation of SLC22A18 expression and it's imprinting mechanism. Methodology: In order to understand the regulation of SLC22A18 expression, we have characterized its promoter. We have used PCR methodology to isolate 5' flanking region of SLC22A18 gene and cloned in pGL3 basic promoter less vector and carried out luciferase assay in HepG2, HEK293 and BeWo cell lines. Results: The isolated sequence has directed a high level of expression of luciferase activity in three human cell lines HepG2, HEK293 and BeWo. Successive 5' and 3' deletion analysis has suggested that a ~198 bp region, from position +78 to -120 from the transcription start site (TSS), contains the core promoter. Interestingly, this region contains no consensus TATA box. However, a 414-bp fragment surrounding the TSS exhibits the characteristics of a CpG island which overlaps with the promoter region. Concluding remarks: Human chromosome 11p15.5 contains several maternally and paternally imprinted genes. SLC22A18 is a paternally imprinted gene belonging to the 11p15.5 region that has been shown to carry mutations in some human neoplasms. Reduced expression of SLC22A18 has been found in hepatoblastomas (HB) and it was proposed that the reduced expression may contribute to HB development. The mechanism of SLC22A18 imprinting is not known. The identification of SLC22A18 promoter will not only help to understand its role in tumorigenesis but it will also help to understand the mechanism of imprinting of this gene. We are in the process of studying the nature and mechanism of genomic imprinting using sodium bisulfite genomic sequencing and chromatin immunoprecipitation techniques.

Item Type:	Conference Poster
Keywords:	SLC22A18;Imprinting;Promoter;ChIP;Bisulfite sequencing
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited:	01 Mar 2005
Last Modified:	19 Sep 2010 04:17
URI:	http://eprints.iisc.ac.in/id/eprint/2519

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