Voltage gated Na+ channels contribute to membrane voltage fluctuation in \alpha T3-1 pituitary gonadotroph cells

Tiwari, JK and Sikdar, SK (1998) Voltage gated Na+ channels contribute to membrane voltage fluctuation in \alpha T3-1 pituitary gonadotroph cells. In: Neuroscience Letters, 242 (3). pp. 167-171.

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Abstract

\alpha T3-1 cells showed a slope resistance of 1.8 G\omega. The cell membrane surface was not smooth and a scanning electron micrograph showed a complex structure with blebs and microvilli like projections. The cells showed spontaneous fluctuations at zero current resting membrane potential and hyperpolarization increased the amplitude of membrane potential fluctuations. The amplitude of membrane potential fluctuations at hyperpolarized membrane potential was attenuated on application of TTX to the bath solution. The potential at which half steady state inactivation of isolated sodium current occurred, was at a very hyperpolarized potential (-95.4 mV). The study presented in this paper shows that the voltage gated sodium channels contribute to the increase in the amplitude of electrical noise with hyperpolarization in \alpha T3-1 cells.

Item Type:	Journal Article
Publication:	Neuroscience Letters
Publisher:	Elsevier science
Additional Information:	Copyright for this article belongs to Elsevier science.
Keywords:	Pituitary gonadotroph; Membrane noise; Potential dependence; Input resistance; Tetrodotoxin; Sodium channel
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	29 Oct 2009 09:51
Last Modified:	19 Sep 2010 05:52
URI:	http://eprints.iisc.ac.in/id/eprint/24852

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