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Endocytosis by random initiation and stabilization of clathrin-coatedpits

Ehrlich, M and Boll, W and Van Oijen, A and Hariharan, R and Chandran, K and Nibert, ML and Kirchhausen, T (2004) Endocytosis by random initiation and stabilization of clathrin-coatedpits. In: Cell, 118 (5). pp. 591-605.

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Official URL: http://dx.doi.org/10.1016/j.cell.2004.08.017


Clathrin-coated vesicles carry traffic from the plasma membrane to endosomes. We report here the real-time visualization of cargo sorting and endocytosis by clathrin-coated pits in living cells. We have detected the formation of coats by monitoring incorporation of fluorescently tagged clathrin or its adaptor AP-2; we have also followed clathrin-mediated uptake of transferrin and of single LDL or reovirus particles. The intensity of a cargo-loaded clathrin cluster grows steadily during its lifetime, and the time required to complete assembly is proportional to the size of the cargo particle. These results are consistent with a nucleation-growth mechanism and an approximately constant growth rate. There are no strongly preferred nucleation sites. A proportion of the nucleation events are weak and short lived. Cargo incorporation occurs primarily or exclusively in a newly formed coated pit. Our data lead to a model in which coated pits initiate randomly but collapse unless stabilized, perhaps by cargo capture.

Item Type: Journal Article
Additional Information: The copyright of this article belongs to Cell Press.
Department/Centre: Division of Electrical Sciences > Computer Science & Automation
Depositing User: Ravi V Nandhan
Date Deposited: 27 Dec 2004
Last Modified: 17 Jan 2012 10:18
URI: http://eprints.iisc.ac.in/id/eprint/2478

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